Uyilo lovavanyo
Izinto zokuvavanya: ubisi olutsha,xylo-oligosaccharides, Lactobacillus bulgaricus (Lb), Streptococcus thermophilus (St).
Ukuqalisa koxinzelelo: Vuselela iLactobacillus bulgaricus kunye neStreptococcus thermophilus kubisi lweskim izihlandlo ezithathu de umsebenzi wazo uzinzile (ujongwa ngovavanyo lwemicroscopic kunye nobisi olugobileyo), uze uzigcine efrijini ukuze zisetyenziswe kamva. Indlela yovavanyo: Lungiselela iisampulu zobisi olutsha ezingama-21 kwaye uzahlule ngokwamaqela ama-3, kunye neesampulu ezisi-7 kwiqela ngalinye. Yongeza i-concentrations (%, w / v) ye-0, 0.5, 0.6, 0.7, 0.8 kwiisampuli kwiqela ngalinye. , 0.9 kunye ne-1.0 i-xylo-oligosaccharides, kwaye udibanise i-Lb kunye ne-St kwimilinganiselo ye-2: 1, 1: 1, kunye ne-1: 2 ngokulandelanayo ukwenza inkcubeko yokuqalisa, kwaye emva koko i-inoculate kumaqela e-3 kunye nenani le-inoculation ye-3%. Khulisa kwiqondo lokushisa elingaguqukiyo lama-42°C, kwaye uthathe iisampulu ngamaxesha aqhelekileyo ukulinganisa inani lebhaktiriya esebenzayo, ixabiso le-pH, ubumuncu kunye nomxholo we-asidi ye-lactic kwiqela ngalinye lobisi.
iziphumo zovavanyo
(1) Lb: St = 2: 1, impembelelo ye-xylo-oligosaccharides ekukhuleni kunye nokuveliswa kwe-asidi ye-yogurt fermentation strains
Njengoko kunokubonwa kwi-Figure 1, i-0 ukuya kwi-1.5 h lixesha lokulungelelaniswa koxinzelelo kunye nenqanaba lokuqala lokungena kwisigaba sokukhula kwe-logarithmic. Uxinzelelo alukhuli ngokukhawuleza, ixabiso le-pH liyancipha ngokukhawuleza, kwaye i-asidi kunye ne-lactic acid yanda ngokukhawuleza. Xa i-xylo-oligosaccharide concentration yi-0.7%, ibhaktheriya iyonke Ixabiso eliphezulu li-1.94×108 CFU/mL (ulawulo yi-1.15×108 CFU / mL), kunye ne-pH, i-acidity kunye ne-lactic acid content ifana nokulawula; phakathi kwe-1.5 h kunye ne-3.5 h, ubunzima bungena kwisigaba sokukhula kwe-logarithmic kwaye sikhula ngokukhawuleza. I-pH yehla ngokukhawuleza, kwaye i-acidity kunye ne-lactic acid content yanda ngokukhawuleza. Xa i-xylo-oligosaccharide yoxinaniso yayiyi-0.7%, inani eliphezulu lebhaktheriya laliyi-5.65.×I-108 CFU / mL (ulawulo lwaluyi-5.41×108 CFU / mL). I-pH, i-acidity, kunye ne-lactic acid content Bayi-5.82, i-63 kunye ne-4.93 ngokulandelanayo, kunye ne-pH, i-acidity kunye ne-lactic acid content yokulawula yayiyi-6.05, 56 kunye ne-4.67. Ukusuka kwiiyure ezi-3.5 ukuya kwiiyure ezi-4.5, inani lebhaktheriya lilonke liye lehla, i-pH yehla ngokucothayo, kwaye i-asidi kunye nomxholo we-asidi ye-lactic wenyuka kancinci.
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